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アイスアフィニティー精製を用いた氷再結晶阻害とアイソレーションの評価による植物の氷結合タンパク質の同定
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Bioquímica
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JoVE Journal Bioquímica
Identification of Plant Ice-binding Proteins Through Assessment of Ice-recrystallization Inhibition and Isolation Using Ice-affinity Purification
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アイスアフィニティー精製を用いた氷再結晶阻害とアイソレーションの評価による植物の氷結合タンパク質の同定

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09:43 min

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May 05, 2017

DOI:

10.3791/55302-v

DOI:
10.3791/55302-v

•

09:43 min
May 05, 2017

•

10 Views
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1Department of Biology,Queen’s University, 2Department of Biomedical and Molecular Sciences,Queen’s University, 3School of Environmental Sciences,Queen’s University

Summary

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この論文では、凍結耐性植物の氷結合タンパク質の同定について、氷再結晶阻害活性の評価とその後の氷親和性精製を用いた天然IBPの単離について概説する。

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Cite this Article

Bredow, M., Tomalty, H. E., Walker, V. K. Identification of Plant Ice-binding Proteins Through Assessment of Ice-recrystallization Inhibition and Isolation Using Ice-affinity Purification. J. Vis. Exp. (123), e55302, doi:10.3791/55302 (2017).

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