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Identification of Plant Ice-binding Proteins Through Assessment of Ice-recrystallization Inhibition and Isolation Using Ice-affinity Purification
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JoVE Journal 生化学
Identification of Plant Ice-binding Proteins Through Assessment of Ice-recrystallization Inhibition and Isolation Using Ice-affinity Purification

Identification of Plant Ice-binding Proteins Through Assessment of Ice-recrystallization Inhibition and Isolation Using Ice-affinity Purification

8,497 Views

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09:43 min

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May 05, 2017

DOI:

10.3791/55302-v

DOI:
10.3791/55302-v

•

09:43 min
May 05, 2017

•

10 Views
, ,
1生物学科,Queen’s University, 2Department of Biomedical and Molecular Sciences,Queen’s University, 3School of Environmental Sciences,Queen’s University

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This paper outlines the identification of ice-binding proteins from freeze-tolerant plants through the assessment of ice-recrystallization inhibition activity and subsequent isolation of native IBPs using ice-affinity purification.

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Ice-binding ProteinsIBPsPlant Freeze ToleranceIce-affinity PurificationIce-recrystallization InhibitionCold-acclimationPlant ExtractProtein ExtractionCentrifugationIce-binding Activity

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Bredow, M., Tomalty, H. E., Walker, V. K. Identification of Plant Ice-binding Proteins Through Assessment of Ice-recrystallization Inhibition and Isolation Using Ice-affinity Purification. J. Vis. Exp. (123), e55302, doi:10.3791/55302 (2017).

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