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JoVE Journal Developmental Biology
Conditional Reprogramming of Pediatric Human Esophageal Epithelial Cells for Use in Tissue Engineering and Disease Investigation
 

Conditional Reprogramming of Pediatric Human Esophageal Epithelial Cells for Use in Tissue Engineering and Disease Investigation

Article DOI: 10.3791/55243-v • 10:15 min • March 22nd, 2017
March 22nd, 2017 •
1, 1, 2, 3
1Department of Pediatrics, UConn Health, 2Department of Gastroenterology, Connecticut Children's Medical Center, 3Department of Surgery, Connecticut Children's Medical Center

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Resumo
Automatic Translation

Expansion of human pediatric esophageal epithelial cells utilizing conditional reprogramming provides investigators with a patient-specific population of cells that can be utilized for engineering esophageal constructs for autologous implantation to treat defects or injury and serve as a reservoir for therapeutic screening assays.

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Conditional Reprogramming Pediatric Human Esophageal Epithelial Cells Tissue Engineering Disease Investigation Esophageal Diseases 3D Esophageal Scaffold Esophageal Tissue Replacement Patient-specific Epithelial Cells Genetic Modifications Eosinophilic Esophagitis Esophageal Atresia Feeder Cells Purity Christopher Foster 3T3 Cells Culture Medium T75 Flasks Trypsin-EDTA
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