Waiting
Processando Login

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Genetics / Rapid Deletion Production in Fungi via
JoVE Journal
Genetics

É necessário ter uma assinatura JoVE para assistir este Faça login ou comece sua avaliação gratuita.

JoVE Journal Genetics
Rapid Deletion Production in Fungi via
 
Click here for the English version

Rapid Deletion Production in Fungi via

Article DOI: 10.3791/55239-v • 11:24 min • June 12th, 2017
June 12th, 2017 •
1, 2, 3, 1
1Toxicology and Mycotoxin Research Unit, NPRC, USDA-ARS, 2Hazera Seeds LTD, Brurim, 3Instituto de Hortofruticultura Subtropical y Mediterránea “La Mayora” - Universidad de Málaga - Consejo Superior de Investigaciones Científicas (IHSM-UMA-CSIC), Estación Experimental “La Mayora”

Capítulos

Resumo
Automatic Translation

Please note that all translations are automatically generated.

Click here for the English version.

Gendeletionsmutanter som alstras genom homolog rekombination är guldstandarden för genfunktionsstudier. OSCAR (One Step Construction av Agrobacterium-Recombination-ready-plasmids) -metoden för snabb generering av deletionskonstruktioner beskrivs. Agrobacterium- medierad svamptransformation följer. Slutligen presenteras en PCR-baserad bekräftelsemetod av gendeletioner i svamptransformanter.

Tags

Genetik Utgåva 124 Svamptransformation Svampdeletionsmutantgenerering ATMT Enstegsbyggande av
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter