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真菌快速缺失生产<em>农杆菌</em>介入OSCAR删除构造的转换

JoVE Journal
Genética

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JoVE Journal Genética

Rapid Deletion Production in Fungi via Agrobacterium Mediated Transformation of OSCAR Deletion Constructs

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真菌快速缺失生产<em>农杆菌</em>介入OSCAR删除构造的转换

9,621 Views

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11:24 min

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June 12, 2017

DOI:

10.3791/55239-v

DOI:

10.3791/55239-v

•

11:24 min

June 12, 2017

•

4 Views

Scott E. Gold, Zahi Paz, María D. García-Pedrajas, Anthony E. Glenn

¹Toxicology and Mycotoxin Research Unit,NPRC, USDA-ARS, ²Hazera Seeds LTD, Brurim, ³Instituto de Hortofruticultura Subtropical y Mediterránea “La Mayora” – Universidad de Málaga – Consejo Superior de Investigaciones Científicas (IHSM-UMA-CSIC), Estación Experimental “La Mayora”

Tags

Rapid Deletion Production Fungi Agrobacterium OSCAR Deletion Constructs Homologous Recombination Functional Genetics Gene Deletion Mutants DelsGate System Primer Design PCR Deletion Confirmation

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