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使用荧光抗体对小鼠表皮石蜡切片的免疫组织化学
JoVE Journal
Biologia
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JoVE Journal Biologia
Immunohistochemistry on Paraffin Sections of Mouse Epidermis Using Fluorescent Antibodies
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使用荧光抗体对小鼠表皮石蜡切片的免疫组织化学

DOI:
10.3791/552-v

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11:27 min

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January 08, 2008

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, , , ,
1Chronic Disease Program,Ottawa Health Research Institute, 2Department of Cellular and Molecular Medicine,Ottawa Health Research Institute

Capítulos

  • 00:00Título
  • 00:32Introduction
  • 01:34Mouse Dissection
  • 03:16Fixation and Paraffin Block Preparation
  • 05:16Dewaxing Paraffin Sections and Antigen Retrieval
  • 07:28Immunohistochemistry
  • 10:33Visualization of Stained Slides
  • 11:01Conclusion

Summary

Tadução automática

Tadução automática

我们描述了我们，在我们的实验室非常常规使用冷冻和石蜡切片修改表皮内的免疫定位可靠的方法。

Tags

ImmunohistochemistryParaffin SectionsMouse EpidermisFluorescent AntibodiesProtein LocalizationBasal LayerSuprabasal LayerStratum Corneum LayerFunctional RoleFrozen SectionsReliable MethodMorphologyAntibody SpecificationsFixation Protocols

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