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Biologia
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Immunohistochemistry on Paraffin Sections of Mouse Epidermis Using Fluorescent Antibodies
JoVE Journal
Biologia
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JoVE Journal Biologia
Immunohistochemistry on Paraffin Sections of Mouse Epidermis Using Fluorescent Antibodies

Immunohistochemistry on Paraffin Sections of Mouse Epidermis Using Fluorescent Antibodies

DOI:
10.3791/552-v

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11:27 min

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January 08, 2008

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, , , ,
1Chronic Disease Program,Ottawa Health Research Institute, 2Department of Cellular and Molecular Medicine,Ottawa Health Research Institute

Capítulos

  • 00:00Título
  • 00:32Introduction
  • 01:34Mouse Dissection
  • 03:16Fixation and Paraffin Block Preparation
  • 05:16Dewaxing Paraffin Sections and Antigen Retrieval
  • 07:28Immunohistochemistry
  • 10:33Visualization of Stained Slides
  • 11:01Conclusion

Summary

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Tadução automática

We describe a reliable method for immunolocalization within the epidermis modified for both frozen and paraffin sections that we use very routinely in our laboratory.

Tags

ImmunohistochemistryParaffin SectionsMouse EpidermisFluorescent AntibodiesProtein LocalizationBasal LayerSuprabasal LayerStratum Corneum LayerFunctional RoleFrozen SectionsReliable MethodMorphologyAntibody SpecificationsFixation Protocols

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