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Immunohistochemistry on Paraffin Sections of Mouse Epidermis Using Fluorescent Antibodies
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Biologie
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JoVE Journal Biologie
Immunohistochemistry on Paraffin Sections of Mouse Epidermis Using Fluorescent Antibodies

Immunohistochemistry on Paraffin Sections of Mouse Epidermis Using Fluorescent Antibodies

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11:27 min

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January 08, 2008

DOI:

10.3791/552-v

DOI:
10.3791/552-v

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11:27 min
January 08, 2008

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5 Views
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1Chronic Disease Program,Ottawa Health Research Institute, 2Department of Cellular and Molecular Medicine,Ottawa Health Research Institute

Summary

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We describe a reliable method for immunolocalization within the epidermis modified for both frozen and paraffin sections that we use very routinely in our laboratory.

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ImmunohistochemistryParaffin SectionsMouse EpidermisFluorescent AntibodiesProtein LocalizationBasal LayerSuprabasal LayerStratum Corneum LayerFunctional RoleFrozen SectionsReliable MethodMorphologyAntibody SpecificationsFixation Protocols

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Cite this Article

Troy, T., Arabzadeh, A., Enikanolaiye, A., Lariviere, N., Turksen, K. Immunohistochemistry on Paraffin Sections of Mouse Epidermis Using Fluorescent Antibodies. J. Vis. Exp. (11), e552, doi:10.3791/552 (2008).

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