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A base Fluorescence-lymphocytaire Assay Convient pour Criblage à haut débit des petites molécules
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
A Fluorescence-based Lymphocyte Assay Suitable for High-throughput Screening of Small Molecules
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A base Fluorescence-lymphocytaire Assay Convient pour Criblage à haut débit des petites molécules

10,182 Views

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08:43 min

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March 10, 2017

DOI:

10.3791/55199-v

DOI:
10.3791/55199-v

•

08:43 min
March 10, 2017

•

4 Views
, , , ,
1Department of Microbiology, Infectiology and Immunology,Université de Montréal, 2Department of Pharmacology and Physiology,Université de Montréal, 3Molecular Biology Program,Université de Montréal

Summary

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Nous présentons dans l'étude d'un nouveau test basé sur la fluorescence en utilisant des lymphocytes dérivés d'une souris transgénique. Ce dosage est adapté pour le criblage à haut débit (HTS) de petites molécules dotées de la capacité de l'une ou l'inhibition de la promotion de l'activation des lymphocytes.

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Cite this Article

Fouda, A., Tahsini, M., Khodayarian, F., Al-nafisah, F., Rafei, M. A Fluorescence-based Lymphocyte Assay Suitable for High-throughput Screening of Small Molecules. J. Vis. Exp. (121), e55199, doi:10.3791/55199 (2017).

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