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实验室规模的生产和治疗性抗体的纯化
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Bioquímica
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JoVE Journal Bioquímica
Laboratory Scale Production and Purification of a Therapeutic Antibody
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实验室规模的生产和治疗性抗体的纯化

DOI:
10.3791/55153-v

•

09:54 min

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January 24, 2017

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, , , ,
1Faculty of Pharmacy,University of Sydney

Capítulos

  • 00:05Título
  • 00:38Stable Transfection of HEK-293 Cells
  • 03:59Large Scale Antibody Production from Batch Overgrowth Culture
  • 05:12Antibody Purification by Affinity Chromatograpy Using an Automated Fast Protein Liquid Chromatography System
  • 07:02Results: Laboratory-scale Production and Purification of Antibody
  • 08:50Conclusion

Summary

Tadução automática

Tadução automática

这个协议描述在哺乳动物表达系统中生产的治疗性抗体的。所描述的方法包括制备载体DNA，稳定转染和人胚肾293细胞系的无血清适应的，设置大规模培养和纯化使用亲和层析组成。

Tags

Antibody ProductionTherapeutic AntibodySerum-free ExpressionHEK-293 CellsStable Cell LineTransfectionPolyethylenimine (PEI)Hygromycin BSerum-free AdaptationPurification

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