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Démêler la fonction d'un effecteur bactérien à partir d'un non-cultivable Pathogen usine utilisant une levure de l'écran à deux hybrides
JoVE Journal
Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Unravelling the Function of a Bacterial Effector from a Non-cultivable Plant Pathogen Using a Yeast Two-hybrid Screen
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Démêler la fonction d'un effecteur bactérien à partir d'un non-cultivable Pathogen usine utilisant une levure de l'écran à deux hybrides

11,102 Views

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11:30 min

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January 20, 2017

DOI:

10.3791/55150-v

DOI:
10.3791/55150-v

•

11:30 min
January 20, 2017

•

2 Views
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1Department of Molecular Biology – Functional Genomics,Laimburg Research Centre

Tags

Bacterial EffectorNon-cultivable Plant PathogenYeast Two-hybrid ScreenCandidatus PhytoplasmaMalus DomesticaApple Proliferation DiseaseInfection ResearchDNA IsolationEffector Gene AmplificationBait VectorSelf-activationEffector ExpressionSD-trp PlateSD-trp MediumNMY51

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Janik, K., Schlink, K. Unravelling the Function of a Bacterial Effector from a Non-cultivable Plant Pathogen Using a Yeast Two-hybrid Screen. J. Vis. Exp. (119), e55150, doi:10.3791/55150 (2017).

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