JoVE Journal
Immunology and Infection
Immunology and Infection
This Conteúdo is Open Access.
Capítulos
Resumo
Bacterial effector proteins are important for establishing successful infections. This protocol describes the experimental identification of proteinaceous binding partners of a bacterial effector protein in its natural plant host. Identifying these effector interactions via yeast two-hybrid screens has become an important tool in unravelling molecular pathogenicity strategies.