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Unravelling the Function of a Bacterial Effector from a Non-cultivable Plant Pathogen Using a Yeast Two-hybrid Screen
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Chapitres
- 00:05Titre
- 00:48Collecting Root and Leaf Samples from Infected Apple Trees
- 02:22Yeast 2 Hybrid Screen
- 07:47Analysis of Clones from Selective Plates
- 08:50Results: Yeast 2 Hybrid to Identify Binding Partners of Bacterial Effector Proteins
- 09:57Conclusion
Bacterial effector proteins are important for establishing successful infections. This protocol describes the experimental identification of proteinaceous binding partners of a bacterial effector protein in its natural plant host. Identifying these effector interactions via yeast two-hybrid screens has become an important tool in unravelling molecular pathogenicity strategies.
