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Immunology and Infection

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JoVE Journal Immunology and Infection
Unravelling the Function of a Bacterial Effector from a Non-cultivable Plant Pathogen Using a Yeast Two-hybrid Screen
 

Unravelling the Function of a Bacterial Effector from a Non-cultivable Plant Pathogen Using a Yeast Two-hybrid Screen

Article DOI: 10.3791/55150-v • 11:30 min • January 20th, 2017
January 20th, 2017 •
1, 1
1Department of Molecular Biology - Functional Genomics, Laimburg Research Centre

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Bacterial effector proteins are important for establishing successful infections. This protocol describes the experimental identification of proteinaceous binding partners of a bacterial effector protein in its natural plant host. Identifying these effector interactions via yeast two-hybrid screens has become an important tool in unravelling molecular pathogenicity strategies.

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Keywords: Bacterial Effector Non-cultivable Plant Pathogen Yeast Two-hybrid Screen Candidatus Phytoplasma Malus Domestica Apple Proliferation Disease Infection Research DNA Isolation Effector Gene Amplification Bait Vector Self-activation Effector Expression SD-trp Plate SD-trp Medium NMY51
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