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用GFP标记TMEM184A构建肝素受体身份确认
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Genética
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JoVE Journal Genética
Using a GFP-tagged TMEM184A Construct for Confirmation of Heparin Receptor Identity
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用GFP标记TMEM184A构建肝素受体身份确认

DOI:
10.3791/55053-v

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10:41 min

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February 17, 2017

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, , ,
1Department of Biological Sciences,Lehigh University, 2Department of Natural Science,DeSales University

Capítulos

  • 00:05Título
  • 00:57Vascular Cell GFP-TMEM184A Expression and Rhodamine-Heparin Binding
  • 03:42Fluorescence Resonance Energy Transfer (FRET) from GFP-TMEM184A to Rhodamine-Heparin, and Live Cell Imaging of Rhodamine-Heparin Uptake
  • 05:15In Vitro Heparin Binding Assay
  • 08:07Results: Representative GFP-TMEM184A-transfection Effects
  • 09:44Conclusion

Summary

Tadução automática

Tadução automática

与羧基末端设计用于真核表达GFP的标签的构建编码TMEM184A，在旨在证实TMEM184A的鉴定如在血管细胞肝素受体测定中使用。

Tags

GFP-taggedTMEM184A ConstructHeparin ReceptorFunctional IdentificationOrphan GenesTransfectionCell CultureRhodamine HeparinConfocal MicroscopyFluorescence Imaging

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