Journal
/
遗传学
/
Using a GFP-tagged TMEM184A Construct for Confirmation of Heparin Receptor Identity
JoVE 杂志
遗传学
需要订阅 JoVE 才能查看此.  登录或开始免费试用。
JoVE 杂志 遗传学
Using a GFP-tagged TMEM184A Construct for Confirmation of Heparin Receptor Identity

Using a GFP-tagged TMEM184A Construct for Confirmation of Heparin Receptor Identity

DOI:
10.3791/55053-v

•

10:41 min

•

February 17, 2017

•

, , ,
1Department of Biological Sciences,Lehigh University, 2Department of Natural Science,DeSales University

Chapters

  • 00:05Title
  • 00:57Vascular Cell GFP-TMEM184A Expression and Rhodamine-Heparin Binding
  • 03:42Fluorescence Resonance Energy Transfer (FRET) from GFP-TMEM184A to Rhodamine-Heparin, and Live Cell Imaging of Rhodamine-Heparin Uptake
  • 05:15In Vitro Heparin Binding Assay
  • 08:07Results: Representative GFP-TMEM184A-transfection Effects
  • 09:44Conclusion

Summary

自动翻译

自动翻译

A construct encoding TMEM184A with a GFP tag at the carboxy-terminus designed for eukaryotic expression, was employed in assays designed to confirm the identification of TMEM184A as a heparin receptor in vascular cells.

Tags

GFP-taggedTMEM184A ConstructHeparin ReceptorFunctional IdentificationOrphan GenesTransfectionCell CultureRhodamine HeparinConfocal MicroscopyFluorescence Imaging

Article

Embed

添加到播放列表

Usage Statistics

Related Videos

Procedure for Adaptive Laboratory Evolution of Microorganisms Using a Chemostat

Detection of Copy Number Alterations Using Single Cell Sequencing

Determination of the Optimal Chromosomal Location(s) for a DNA Element in Escherichia coli Using a Novel Transposon-mediated Approach

High-resolution Melting PCR for Complement Receptor 1 Length Polymorphism Genotyping: An Innovative Tool for Alzheimer’s Disease Gene Susceptibility Assessment

A Method to Study the C924T Polymorphism of the Thromboxane A2 Receptor Gene

An Ecdysone Receptor-based Singular Gene Switch for Deliberate Expression of Transgene with Robustness, Reversibility, and Negligible Leakiness

High-throughput Identification of Gene Regulatory Sequences Using Next-generation Sequencing of Circular Chromosome Conformation Capture (4C-seq)

Endogenous Protein Tagging in Human Induced Pluripotent Stem Cells Using CRISPR/Cas9

qKAT: Quantitative Semi-automated Typing of Killer-cell Immunoglobulin-like Receptor Genes

Mass Spectrometry-Based Proteomics Analyses Using the OpenProt Database to Unveil Novel Proteins Translated from Non-Canonical Open Reading Frames

Read Article