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Using a GFP-tagged TMEM184A Construct for Confirmation of Heparin Receptor Identity
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Using a GFP-tagged TMEM184A Construct for Confirmation of Heparin Receptor Identity

Using a GFP-tagged TMEM184A Construct for Confirmation of Heparin Receptor Identity

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10:41 min

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February 17, 2017

DOI:

10.3791/55053-v

DOI:
10.3791/55053-v

•

10:41 min
February 17, 2017

•

2 Views
, , ,
1Department of Biological Sciences,Lehigh University, 2Department of Natural Science,DeSales University

Summary

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A construct encoding TMEM184A with a GFP tag at the carboxy-terminus designed for eukaryotic expression, was employed in assays designed to confirm the identification of TMEM184A as a heparin receptor in vascular cells.

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GFP-taggedTMEM184A ConstructHeparin ReceptorFunctional IdentificationOrphan GenesTransfectionCell CultureRhodamine HeparinConfocal MicroscopyFluorescence Imaging

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Farwell, S. L. N., Slee, J. B., Li, Y., Lowe-Krentz, L. J. Using a GFP-tagged TMEM184A Construct for Confirmation of Heparin Receptor Identity. J. Vis. Exp. (120), e55053, doi:10.3791/55053 (2017).

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