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Establishment of Proliferative Tetraploid Cells from Nontransformed Human Fibroblasts
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Establishment of Proliferative Tetraploid Cells from Nontransformed Human Fibroblasts
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January 08, 2017
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Summary
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Although proliferative polyploid cells are necessary to analyze chromosomal instability of polyploid cells, creating such cells from nontransformed human cells is not easy. The present report describes relatively simple procedures to establish proliferative tetraploid cells free of a diploid population from normal human fibroblasts.
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Ohshima, S., Seyama, A. Establishment of Proliferative Tetraploid Cells from Nontransformed Human Fibroblasts. J. Vis. Exp. (119), e55028, doi:10.3791/55028 (2017).
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