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إنشاء خلايا التكاثري رباعي الصيغة الصبغية من Nontransformed الخلايا الليفية الإنسان
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إنشاء خلايا التكاثري رباعي الصيغة الصبغية من Nontransformed الخلايا الليفية الإنسان
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January 08, 2017
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Summary
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Although proliferative polyploid cells are necessary to analyze chromosomal instability of polyploid cells, creating such cells from nontransformed human cells is not easy. The present report describes relatively simple procedures to establish proliferative tetraploid cells free of a diploid population from normal human fibroblasts.
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Cite this Article
Ohshima, S., Seyama, A. Establishment of Proliferative Tetraploid Cells from Nontransformed Human Fibroblasts. J. Vis. Exp. (119), e55028, doi:10.3791/55028 (2017).
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