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Эффективный метод для выделения высокоочищенного РНК из семян для использования в количественном анализе Транскриптом
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Bioquímica
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JoVE Journal Bioquímica
An Efficient Method for the Isolation of Highly Purified RNA from Seeds for Use in Quantitative Transcriptome Analysis
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Эффективный метод для выделения высокоочищенного РНК из семян для использования в количественном анализе Транскриптом

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06:31 min

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January 11, 2017

DOI:

10.3791/55008-v

DOI:
10.3791/55008-v

•

06:31 min
January 11, 2017

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3 Views
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1Laboratory of Biological Diversity, Department of Evolutionary Biology and Biodiversity,National Institute for Basic Biology, 2Department of Basic Biology,SOKENDAI (The Graduate University for Advanced Studies), 3Department of Cell Biology,National Institute for Basic Biology

Tags

RNA ExtractionSeedQuantitative Transcriptome AnalysisArabidopsis ThalianaPVPCell Lysis BufferCentrifugationHomogenizationSpin Column-based MethodHigh-purity RNACereal BiologyGene ProfilesTranscripts

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Kanai, M., Mano, S., Nishimura, M. An Efficient Method for the Isolation of Highly Purified RNA from Seeds for Use in Quantitative Transcriptome Analysis. J. Vis. Exp. (119), e55008, doi:10.3791/55008 (2017).

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