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Culture of Embryonic Mouse Cochlear Explants and Gene Transfer by Electroporation
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Culture of Embryonic Mouse Cochlear Explants and Gene Transfer by Electroporation
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January 12, 2015
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We present a method that describes isolation and culture of cochlear explants from embryonic mouse inner ear. We also demonstrate a method for gene transfer into cochlear explants via square-wave electroporation. The in vitro explant culture coupled with gene transfer technique enables researchers to study the effects of altering gene expression during development.
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Haque, K. D., Pandey, A. K., Kelley, M. W., Puligilla, C. Culture of Embryonic Mouse Cochlear Explants and Gene Transfer by Electroporation. J. Vis. Exp. (95), e52260, doi:10.3791/52260 (2015).
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