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Kondrogena Differentiering Induktion av Fett härrörande stamceller genom Radial Gravity
JoVE Journal
Biologia do Desenvolvimento
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JoVE Journal Biologia do Desenvolvimento
Chondrogenic Differentiation Induction of Adipose-derived Stem Cells by Centrifugal Gravity
DOI:

08:30 min

February 24, 2017

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Capítulos

  • 00:05Título
  • 00:54Centrifugal Gravity Loading
  • 02:22Pellet Culture
  • 03:12Micromass Culture
  • 04:10Paraffin-embedding Cell Pellets
  • 05:59Results: Upregulation of Chondrogenic Differentiation Markers in Adipose-derived Stem Cells Following Centrifugal Gravity Loading
  • 07:59Conclusion

Summary

Tadução automática

Mechanical stress can induce the chondrogenic differentiation of stem cells, providing a potential therapeutic approach for the repair of impaired cartilage. We present a protocol to induce the chondrogenic differentiation of adipose-derived stem cells (ASCs) using centrifugal gravity (CG). CG-induced upregulation of SOX9 results in the development of chondrogenic phenotypes.

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