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检查蛋白酶体大会与重组古菌蛋白酶体和非变性PAGE：该案例相结合的办法
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JoVE Journal Bioquímica
Examining Proteasome Assembly with Recombinant Archaeal Proteasomes and Nondenaturing PAGE: The Case for a Combined Approach
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检查蛋白酶体大会与重组古菌蛋白酶体和非变性PAGE：该案例相结合的办法

DOI:
10.3791/54860-v

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09:57 min

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December 17, 2016

•

,
1Department of Biology,Indiana University-Purdue University, Indianapolis (IUPUI)

Capítulos

  • 00:05Título
  • 00:43Bacterial Lysis and Lysate Mixing
  • 02:31Protein Purification via Immobilized Cobalt Affinity Resin (ICAR)
  • 05:04Nondenaturing Polyacrylamide Gel Electrophoresis (PAGE)
  • 08:09Results: A Combined Approach for Assaying Proteasome Assembly
  • 09:24Conclusion

Summary

Tadução automática

Tadução automática

该协议使用两个亚基共表达和postlysis亚基重组蛋白酶组件的更彻底的检查混合。

Tags

Proteasome AssemblyRecombinant Archaeal ProteasomesNondenaturing PAGEAssembly IntermediatesEukaryotic ProteasomeArchaeal 20S ProteasomesCoexpressionLysate MixingProtein Complex Assembly

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