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Examining Proteasome Assembly with Recombinant Archaeal Proteasomes and Nondenaturing PAGE: The Case for a Combined Approach
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Biochemie
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JoVE Journal Biochemie
Examining Proteasome Assembly with Recombinant Archaeal Proteasomes and Nondenaturing PAGE: The Case for a Combined Approach

Examining Proteasome Assembly with Recombinant Archaeal Proteasomes and Nondenaturing PAGE: The Case for a Combined Approach

DOI:
10.3791/54860-v

•

09:57 min

•

December 17, 2016

•

,
1Department of Biology,Indiana University-Purdue University, Indianapolis (IUPUI)

Kapitel

  • 00:05Titel
  • 00:43Bacterial Lysis and Lysate Mixing
  • 02:31Protein Purification via Immobilized Cobalt Affinity Resin (ICAR)
  • 05:04Nondenaturing Polyacrylamide Gel Electrophoresis (PAGE)
  • 08:09Results: A Combined Approach for Assaying Proteasome Assembly
  • 09:24Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

This protocol uses both subunit coexpression and postlysis subunit mixing for a more thorough examination of recombinant proteasome assembly.

Tags

Proteasome AssemblyRecombinant Archaeal ProteasomesNondenaturing PAGEAssembly IntermediatesEukaryotic ProteasomeArchaeal 20S ProteasomesCoexpressionLysate MixingProtein Complex Assembly

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