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Examining Proteasome Assembly with Recombinant Archaeal Proteasomes and Nondenaturing PAGE: The Case for a Combined Approach
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生物化学
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JoVE 杂志 生物化学
Examining Proteasome Assembly with Recombinant Archaeal Proteasomes and Nondenaturing PAGE: The Case for a Combined Approach

Examining Proteasome Assembly with Recombinant Archaeal Proteasomes and Nondenaturing PAGE: The Case for a Combined Approach

DOI:
10.3791/54860-v

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09:57 min

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December 17, 2016

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1Department of Biology,Indiana University-Purdue University, Indianapolis (IUPUI)

Chapters

  • 00:05Title
  • 00:43Bacterial Lysis and Lysate Mixing
  • 02:31Protein Purification via Immobilized Cobalt Affinity Resin (ICAR)
  • 05:04Nondenaturing Polyacrylamide Gel Electrophoresis (PAGE)
  • 08:09Results: A Combined Approach for Assaying Proteasome Assembly
  • 09:24Conclusion

Summary

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This protocol uses both subunit coexpression and postlysis subunit mixing for a more thorough examination of recombinant proteasome assembly.

Tags

Proteasome AssemblyRecombinant Archaeal ProteasomesNondenaturing PAGEAssembly IntermediatesEukaryotic ProteasomeArchaeal 20S ProteasomesCoexpressionLysate MixingProtein Complex Assembly

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