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Eencellige genexpressie met behulp van Multiplex RT-qPCR heterogeniteit van Rare lymfoïde populaties karakteriseren
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Genética
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JoVE Journal Genética
Single-cell Gene Expression Using Multiplex RT-qPCR to Characterize Heterogeneity of Rare Lymphoid Populations
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Eencellige genexpressie met behulp van Multiplex RT-qPCR heterogeniteit van Rare lymfoïde populaties karakteriseren

10,746 Views

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10:23 min

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January 19, 2017

DOI:

10.3791/54858-v

DOI:
10.3791/54858-v

•

10:23 min
January 19, 2017

•

4 Views
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1Unit for Lymphopoieseis, Immunology Department, INSERM U1223, University Paris Diderot, Sorbonne Paris Cité, Cellule Pasteur,Institut Pasteur, 2Center for Translational Science,Institut Pasteur, INSERM UMS20

Summary

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Dit protocol beschrijft hoe de expressie van een groot scala van genen op het klonale niveau te beoordelen. Single-cell RT-qPCR levert zeer betrouwbare resultaten met een sterke gevoeligheid voor honderden monsters en genen.

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Cite this Article

Perchet, T., Chea, S., Hasan, M., Cumano, A., Golub, R. Single-cell Gene Expression Using Multiplex RT-qPCR to Characterize Heterogeneity of Rare Lymphoid Populations. J. Vis. Exp. (119), e54858, doi:10.3791/54858 (2017).

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