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单细胞基因表达利用多重RT-qPCR的表征罕见的淋巴人群的异质性

JoVE Journal
Genética

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JoVE Journal Genética

Single-cell Gene Expression Using Multiplex RT-qPCR to Characterize Heterogeneity of Rare Lymphoid Populations

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单细胞基因表达利用多重RT-qPCR的表征罕见的淋巴人群的异质性

10,746 Views

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10:23 min

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January 19, 2017

DOI:

10.3791/54858-v

DOI:

10.3791/54858-v

•

10:23 min

January 19, 2017

•

4 Views

Thibaut Perchet¹, Sylvestre Chea¹, Milena Hasan², Ana Cumano¹, Rachel Golub¹

¹Unit for Lymphopoieseis, Immunology Department, INSERM U1223, University Paris Diderot, Sorbonne Paris Cité, Cellule Pasteur,Institut Pasteur, ²Center for Translational Science,Institut Pasteur, INSERM UMS20

Summary

这个协议描述如何评估在克隆水平大阵列的基因的表达。单细胞RT-qPCR的产生与数百个样品和基因的敏感性较强高度可靠的结果。

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