Journal
/
Genética
/
Optogenetische willekeurige mutagenese met behulp van histon-miniSOG in<em> C. elegans</em
JoVE Journal
Genética
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Genética
Optogenetic Random Mutagenesis Using Histone-miniSOG in C. elegans
Please note that all translations are automatically generated. Click here for the English version.

Optogenetische willekeurige mutagenese met behulp van histon-miniSOG in<em> C. elegans</em

8,880 Views

•

04:51 min

•

November 14, 2016

DOI:

10.3791/54810-v

DOI:
10.3791/54810-v

•

04:51 min
November 14, 2016

•

2 Views
,
1Division of Biological Sciences, Section of Neurobiology,University of California in San Diego and Howard Hughes Medical Institute, 2Department of Cellular and Molecular Medicine,University of California in San Diego School of Medicine and Howard Hughes Medical Institute

Summary

Automatically generated

Genetisch gecodeerde histon-miniSOG induceert genoom-brede erfelijke mutaties in een blauw licht-afhankelijke manier. Dit mutagenese methode is eenvoudig, snel, vrij van giftige chemicaliën, en zeer geschikt voor forward genetische screening en transgene integratie.

Explore More Videos

OptogeneticRandom MutagenesisHistone-miniSOGC. ElegansLEDReactive Oxygen SpeciesROS-generating ProteinsMutagenesis ProtocolBlue LightDigital Function GeneratorPhotometerCopper ChlorideSine WaveYoung Adult Hermaphrodites

Read Article

Cite this Article

Noma, K., Jin, Y. Optogenetic Random Mutagenesis Using Histone-miniSOG in C. elegans. J. Vis. Exp. (117), e54810, doi:10.3791/54810 (2016).

Download .ris file

Copy

Share Video

.

Copy