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Optogenetic Tilfældig mutagenese ved anvendelse histon-miniSOG i<em> C. elegans</em
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Genética
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JoVE Journal Genética
Optogenetic Random Mutagenesis Using Histone-miniSOG in C. elegans

Optogenetic Tilfældig mutagenese ved anvendelse histon-miniSOG i<em> C. elegans</em

8,880 Views

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04:51 min

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November 14, 2016

DOI:

10.3791/54810-v

DOI:
10.3791/54810-v

•

04:51 min
November 14, 2016

•

2 Views
,
1Division of Biological Sciences, Section of Neurobiology,University of California in San Diego and Howard Hughes Medical Institute, 2Department of Cellular and Molecular Medicine,University of California in San Diego School of Medicine and Howard Hughes Medical Institute

Summary

Automatically generated

Genetisk kodet histon-miniSOG inducerer genom-dækkende arvelige mutationer i et blåt lys-afhængig måde. Denne mutagenese metode er enkel, hurtig, fri for giftige kemikalier, og velegnet til frem genetisk screening og transgen integration.

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Cite this Article

Noma, K., Jin, Y. Optogenetic Random Mutagenesis Using Histone-miniSOG in C. elegans. J. Vis. Exp. (117), e54810, doi:10.3791/54810 (2016).

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