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Biologia do Desenvolvimento
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Analysis of Coronary Vessels in Cleared Embryonic Hearts
JoVE Journal
Biologia do Desenvolvimento
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JoVE Journal Biologia do Desenvolvimento
Analysis of Coronary Vessels in Cleared Embryonic Hearts

Analysis of Coronary Vessels in Cleared Embryonic Hearts

DOI:
10.3791/54800-v

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08:25 min

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December 07, 2016

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, , ,
1Developmental Biology of Birth Defects,UCL Institute of Child Health, 2MRC Centre for Regenerative Medicine, SCRM Building,University of Edinburgh

Capítulos

  • 00:05Título
  • 00:51Embryonic Heart Dissection and Fixation
  • 02:07Embryonic Heart Whole Mount Immunostaining
  • 05:25Results: Representative Embryonic Heart Imaging
  • 07:11Conclusion

Summary

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We present a protocol for the analysis of coronary vessels in whole embryonic murine hearts up to E15.5, using standard immunological staining methods followed by optical clearance and confocal microscopy. This technique enables visualization of blood vessels throughout the entire heart without the need for time-consuming analysis of serial sections.

Tags

Coronary VesselsBABBCardiovascular DevelopmentCoronary Heart DiseaseEmbryonic HeartsImmunostainingWhole MountPrimary Antibody

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