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方法来检查淋巴腺和血细胞中<em>果蝇</em>幼虫

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Biologia do Desenvolvimento

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JoVE Journal Biologia do Desenvolvimento

Methods to Examine the Lymph Gland and Hemocytes in Drosophila Larvae

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方法来检查淋巴腺和血细胞中<em>果蝇</em>幼虫

DOI:

10.3791/54544-v

•

11:49 min

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November 28, 2016

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Theresa A. Reimels², Cathie M. Pfleger²

¹The Graduate School of Biomedical Sciences,The Icahn School of Medicine at Mount Sinai, ²Department of Oncological Sciences,The Icahn School of Medicine at Mount Sinai

Capítulos

00:05Título
00:44Hemolymph Collection to Determine Concentration
01:45Hemolymph Collection and Immunochemistry
04:32Larval Lymph Gland Dissection
06:31Antibody Staining of Larval Lymph Glands
08:32Mounting Lymph Gland Tissue for Microscopy
09:32Results: Quantification and Imaging of Hemocytes, and Imaging of Dissected and Immunostained Lymph Gland Tissue
11:19Conclusion

Summary

Tadução automática

果蝇和哺乳动物造血系统有许多共同的特点，使果蝇有吸引力的遗传模型来研究造血功能。这里，我们证明了免疫组化的主要造血幼虫器官解剖和安装。我们还描述的方法来测定各种幼虫造血车厢，包括循环血细胞和无柄水晶细胞。

Tags

Keywords: Lymph Gland Hemocytes Drosophila Larvae Hematopoietic System Immunohistochemistry Hemolymph Collection Hemocytometer Hemolymph Immunochemistry Fixation Permeabilization Primary Antibody

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