This protocol shows how to perform cytoplasmic microinjection in farm animal zygotes. This technique can be used to deliver any solution into the one-cell embryo such as genome editing tools to generate knockout animals.
Bogliotti, Y. S., Vilarino, M., Ross, P. J. Laser-assisted Cytoplasmic Microinjection in Livestock Zygotes. J. Vis. Exp. (116), e54465, doi:10.3791/54465 (2016).