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Laser-assisted Cytoplasmic Microinjection in Livestock Zygotes
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JoVE Journal Genética
Laser-assisted Cytoplasmic Microinjection in Livestock Zygotes

Laser-assisted Cytoplasmic Microinjection in Livestock Zygotes

DOI:
10.3791/54465-v

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08:59 min

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October 05, 2016

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, ,
1Department of Animal Science,University of California, Davis

Capítulos

  • 00:05Título
  • 00:42Formation of the Injection Micropipette
  • 02:19Formation of the Holding Micropipette
  • 03:29Micromanipulator Setup
  • 04:03Preparation of the Injection Dish
  • 04:43Microinjection
  • 07:30Results: High Success Rate with Laser-assisted Cytoplasmic Microinjection
  • 08:19Conclusion

Summary

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This protocol shows how to perform cytoplasmic microinjection in farm animal zygotes. This technique can be used to deliver any solution into the one-cell embryo such as genome editing tools to generate knockout animals.

Tags

Keywords: Laser-assisted MicroinjectionCytoplasmic MicroinjectionLivestock ZygotesGenome EditingCRISPR Cas9Transgenic AnimalsBorosilicate Glass CapillaryMicropipette PullerMicroforgePipette DiameterPipette Taper

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