Journal
/
Neurociência
/
Opplisting av Neural stamceller Bruke Klonale Analyser
JoVE Journal
Neurociência
This content is Free Access.
JoVE Journal Neurociência
Enumeration of Neural Stem Cells Using Clonal Assays

Opplisting av Neural stamceller Bruke Klonale Analyser

DOI:
10.3791/54456-v

•

10:32 min

•

October 04, 2016

•

, , , , , , ,
1Neural Stem Cell Laboratory, Institute of Medical Biology,Agency for Science, Technology and Research (A*STAR)

Capítulos

  • 00:05Título
  • 00:50Culture of Clonal Sample Neurospheres
  • 02:07Preparation of Neurosphere-conditioned Medium
  • 04:20Transfer of Sample Neurospheres to the 50-well-chambered Coverslip
  • 06:25Differentiation of Sample Neurospheres
  • 07:18Fixing the Differentiated Neurospheres
  • 08:16Results: Differentiation of Clonal Neurospheres and Enumeration of NSC Frequency
  • 09:28Conclusion

Summary

Tadução automática

Tadução automática

Neural stamceller (NSC) refererer til celler som kan fornye seg selv og skille ut de tre nevrale linjene. Her beskriver vi en protokoll for å bestemme NSC frekvens i en gitt cellepopulasjon ved bruk av neurosfære dannelse og differensiering i henhold klonale betingelser.

Tags

Keywords: Neural Stem CellsClonal AssaysNeurosphere FormationNeural Stem Cell MarkersNeural ProgenitorsE14.5 MouseNSC Growth MediumTrypan-blue StainingHemocytometerPoly-L-lysineLamininNSC Differentiation Medium

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

check_url/pt/54456?article_type=v

Growth and Differentiation of Adult Hippocampal Arctic Ground Squirrel Neural Stem Cells
check_url/pt/54456?article_type=v

Isolation and Expansion of the Adult Mouse Neural Stem Cells Using the Neurosphere Assay
check_url/pt/54456?article_type=v

Neural-Colony Forming Cell Assay: An Assay To Discriminate Bona Fide Neural Stem Cells from Neural Progenitor Cells
check_url/pt/54456?article_type=v

Generation of Neural Stem Cells from Discarded Human Fetal Cortical Tissue
check_url/pt/54456?article_type=v

Isolasjon og kultur av neural crest stamceller fra Human Hair Follikler
check_url/pt/54456?article_type=v

Den Neuroblast analysen: en analyse for generering og berikelse av neuronal stamceller fra Skille Neural Stem Cell familie Bruke flowcytometrisystemer
check_url/pt/54456?article_type=v

Isolering og kultur neural crest Celler fra Embryonic murin Neural Tube
check_url/pt/54456?article_type=v

Feeder-free Utledning av Neural Crest stamceller fra menneskelige pluripotent stamceller
check_url/pt/54456?article_type=v

Utvikling av en raffinert protokoll for Trans-Innbukking Subretinal transplantasjon av menneskelig netthinnens Pigment epitelceller i rotte øyne
check_url/pt/54456?article_type=v

Isolasjon og kultur av embryonale mus embryonale stamceller

Read Article