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Fluorescentie Activated Cell Sorting (FACS) en genexpressie analyse van Fos expressie neuronen van verse en diepgevroren Rat Brain Tissue
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Neurociência
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JoVE Journal Neurociência
Fluorescence Activated Cell Sorting (FACS) and Gene Expression Analysis of Fos-expressing Neurons from Fresh and Frozen Rat Brain Tissue
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Fluorescentie Activated Cell Sorting (FACS) en genexpressie analyse van Fos expressie neuronen van verse en diepgevroren Rat Brain Tissue

28,473 Views

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08:37 min

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August 27, 2016

DOI:

10.3791/54358-v

DOI:
10.3791/54358-v

•

08:37 min
August 27, 2016

•

35 Views
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1Behavioral Neuroscience Research Branch, Intramural Research Program,NIDA, NIH, DHHS, 2Division of Rheumatology, School of Medicine,Johns Hopkins University

Summary

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Hier presenteren we een Fluorescence Activated Cell Sorting (FACS) protocol om moleculaire veranderingen studeren in Fos-uiting van neuronale ensembles van zowel verse als bevroren hersenweefsel. Het gebruik van bevroren weefsel maakt FACS isoleren van vele hersengebieden over meerdere zittingen aan het gebruik van kostbare proefdieren maximaliseren.

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Cite this Article

Rubio, F. J., Li, X., Liu, Q., Cimbro, R., Hope, B. T. Fluorescence Activated Cell Sorting (FACS) and Gene Expression Analysis of Fos-expressing Neurons from Fresh and Frozen Rat Brain Tissue. J. Vis. Exp. (114), e54358, doi:10.3791/54358 (2016).

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