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A Detailed Protocol for Characterizing the Murine C1498 Cell Line and its Associated Leukemia Mouse Model
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A Detailed Protocol for Characterizing the Murine C1498 Cell Line and its Associated Leukemia Mouse Model
Capítulos
- 00:05Título
- 00:53Intravenous C1498 Injection and Retro-orbital Blood Collection
- 02:15Blood Mononuclear Cells Isolation
- 03:11Bone Marrow Cells Isolation
- 04:41Bone Marrow Cells Preparation on Slides and May-Grünwald Giemsa staining
- 06:06Representative Results: C1498 Acute Myeloid Leukemia Characterization
- 07:14Conclusion
This manuscript provides a technical procedure that can be used to characterize C1498 cell cultures in vitro and the acute leukemia induced in mice after their injection. Phenotypic and functional analyses are performed using flow cytometry, immunofluorescence microscopy, cytochemistry and May-Grünwald Giemsa staining.
