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A Detailed Protocol for Characterizing the Murine C1498 Cell Line and its Associated Leukemia Mouse Model
JoVE 杂志
癌症研究
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JoVE 杂志 癌症研究
A Detailed Protocol for Characterizing the Murine C1498 Cell Line and its Associated Leukemia Mouse Model

A Detailed Protocol for Characterizing the Murine C1498 Cell Line and its Associated Leukemia Mouse Model

19,164 Views

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08:00 min

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October 14, 2016

DOI:

10.3791/54270-v

DOI:
10.3791/54270-v

•

08:00 min
October 14, 2016

•

36 Views
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1Institut pour la Recherche sur le Cancer de Lille (IRCL),INSERM, UMR-S-1172, Centre de Recherche Jean-Pierre Aubert (JPARC), 2Université de Lille

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This manuscript provides a technical procedure that can be used to characterize C1498 cell cultures in vitro and the acute leukemia induced in mice after their injection. Phenotypic and functional analyses are performed using flow cytometry, immunofluorescence microscopy, cytochemistry and May-Grünwald Giemsa staining.

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Mopin, A., Driss, V., Brinster, C. A Detailed Protocol for Characterizing the Murine C1498 Cell Line and its Associated Leukemia Mouse Model. J. Vis. Exp. (116), e54270, doi:10.3791/54270 (2016).

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