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Een stap Negatieve Chromatografische zuivering van<em> Helicobacter pylori</em> Neutrofiel-activerend eiwit overexpressie in<em> Escherichia coli</em> In Batchmodus
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
One-step Negative Chromatographic Purification of Helicobacter pylori Neutrophil-activating Protein Overexpressed in Escherichia coli in Batch Mode
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Een stap Negatieve Chromatografische zuivering van<em> Helicobacter pylori</em> Neutrofiel-activerend eiwit overexpressie in<em> Escherichia coli</em> In Batchmodus

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10:44 min

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June 18, 2016

DOI:

10.3791/54043-v

DOI:
10.3791/54043-v

•

10:44 min
June 18, 2016

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1 Views
, , , ,
1Institute of Molecular and Cellular Biology,National Tsing Hua University, 2Department of Life Science,National Tsing Hua University

Tags

Helicobacter PyloriNeutrophil-activating ProteinHP-NAPEscherichia ColiNegative Chromatographic PurificationBatch ModeDEAE Ion Exchange ResinProtein PurificationAggregation PreventionBradford Assay

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Kuo, T., Hong, Z., Tsai, C., Yang, Y., Fu, H. One-step Negative Chromatographic Purification of Helicobacter pylori Neutrophil-activating Protein Overexpressed in Escherichia coli in Batch Mode. J. Vis. Exp. (112), e54043, doi:10.3791/54043 (2016).

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