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One-step Negative Chromatographic Purification of Helicobacter pylori Neutrophil-activating Protein Overexpressed in Escherichia coli in Batch Mode
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JoVE Journal 免疫学と感染
One-step Negative Chromatographic Purification of Helicobacter pylori Neutrophil-activating Protein Overexpressed in Escherichia coli in Batch Mode

One-step Negative Chromatographic Purification of Helicobacter pylori Neutrophil-activating Protein Overexpressed in Escherichia coli in Batch Mode

9,584 Views

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10:44 min

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June 18, 2016

DOI:

10.3791/54043-v

DOI:
10.3791/54043-v

•

10:44 min
June 18, 2016

•

1 Views
, , , ,
1Institute of Molecular and Cellular Biology,National Tsing Hua University, 2Department of Life Science,National Tsing Hua University

概要

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A high yield method for one-step negative purification of recombinant Helicobacter pylori neutrophil-activating protein (HP-NAP) overexpressed in Escherichia coli by using diethylaminoethyl resins in batch mode is described. HP-NAP purified by this method is beneficial for the development of vaccines, drugs, or diagnostics for H. pylori-associated diseases.

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Helicobacter PyloriNeutrophil-activating ProteinHP-NAPEscherichia ColiNegative Chromatographic PurificationBatch ModeDEAE Ion Exchange ResinProtein PurificationAggregation PreventionBradford Assay

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Cite this Article

Kuo, T., Hong, Z., Tsai, C., Yang, Y., Fu, H. One-step Negative Chromatographic Purification of Helicobacter pylori Neutrophil-activating Protein Overexpressed in Escherichia coli in Batch Mode. J. Vis. Exp. (112), e54043, doi:10.3791/54043 (2016).

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