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Immunology and Infection

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JoVE Journal Immunology and Infection
One-step Negative Chromatographic Purification of Helicobacter pylori Neutrophil-activating Protein Overexpressed in Escherichia coli in Batch Mode
 

One-step Negative Chromatographic Purification of Helicobacter pylori Neutrophil-activating Protein Overexpressed in Escherichia coli in Batch Mode

Article DOI: 10.3791/54043-v • 10:44 min • June 18th, 2016
June 18th, 2016 •
1, 1, 1, 1, 1,2
1Institute of Molecular and Cellular Biology, National Tsing Hua University, 2Department of Life Science, National Tsing Hua University

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A high yield method for one-step negative purification of recombinant Helicobacter pylori neutrophil-activating protein (HP-NAP) overexpressed in Escherichia coli by using diethylaminoethyl resins in batch mode is described. HP-NAP purified by this method is beneficial for the development of vaccines, drugs, or diagnostics for H. pylori-associated diseases.

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Negative Chromatographic Purification Helicobacter Pylori Neutrophil-activating Protein HP-NAP Escherichia Coli Batch Mode Di-ethyl Amino-ethyl Ion Exchange Resin Native Form High Purity High Yield Batch Binding Procedure DEAE Resin Centrifugation Of Gravity Flow Prevention Treatment Prognostic Cancer Therapy Immunogenic Properties Immunomodulatory Properties Sterilization
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