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Immunomagnetische Scheiding van Fat Depot-specifieke SCA1<sup> hoog</sup>-Vetcellen afgeleide stamcellen (ASC)
JoVE Journal
Biologia do Desenvolvimento
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JoVE Journal Biologia do Desenvolvimento
Immunomagnetic Separation of Fat Depot-specific Sca1high Adipose-derived Stem Cells (ASCs)

Immunomagnetische Scheiding van Fat Depot-specifieke SCA1<sup> hoog</sup>-Vetcellen afgeleide stamcellen (ASC)

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08:52 min

August 11, 2016

DOI:

08:52 min
August 11, 2016

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Summary

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We present the techniques required to isolate the stromal vascular fraction (SVF) from mouse inguinal (subcutaneous) and perigonadal (visceral) adipose tissue depots to assess their gene expression and collagenolytic activity. This method includes the enrichment of Sca1high adipose-derived stem cells (ASCs) using immunomagnetic cell separation.

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