Journal
/
Biología del desarrollo
/
Immunomagnetic Separation of Fat Depot-specific Sca1high Adipose-derived Stem Cells (ASCs)
JoVE Revista
Biología del desarrollo
Se requiere una suscripción a JoVE para ver este contenido.  Inicie sesión o comience su prueba gratuita.
JoVE Revista Biología del desarrollo
Immunomagnetic Separation of Fat Depot-specific Sca1high Adipose-derived Stem Cells (ASCs)

Immunomagnetic Separation of Fat Depot-specific Sca1high Adipose-derived Stem Cells (ASCs)

DOI:
10.3791/53890-v

•

08:52 min

•

August 11, 2016

•

,
1Department of Internal Medicine, Division of Metabolism, Endocrinology and Diabetes,Biointerfaces Institute, University of Michigan

Capítulos

  • 00:05Título
  • 00:45Subcutaneous (SQ) and Visceral (VIS) Fat Pad Isolation
  • 02:35Fat Pad Collagenase Digestion
  • 04:20Magnetic Cell Separation
  • 06:31Results: Fat Depot-dependent Expression of Collagenolytic Matrix Metalloproteinases (MMPs)
  • 07:52Conclusion

Summary

Traducción Automática

Traducción Automática

We present the techniques required to isolate the stromal vascular fraction (SVF) from mouse inguinal (subcutaneous) and perigonadal (visceral) adipose tissue depots to assess their gene expression and collagenolytic activity. This method includes the enrichment of Sca1high adipose-derived stem cells (ASCs) using immunomagnetic cell separation.

Tags

Immunomagnetic SeparationSca1high Adipose-derived Stem CellsFat Depot-specificMesenchymal Stem CellsSubcutaneous FatVisceral FatCollagenaseCell Sorting

Article

Embed

Añadir a la lista de reproducción

Usage Statistics

Videos relacionados

Isolation and Enrichment of Human Adipose-derived Stromal Cells for Enhanced Osteogenesis

Isolation and Differentiation of Adipose-Derived Stem Cells from Porcine Subcutaneous Adipose Tissues

An Efficient Method to Obtain Dedifferentiated Fat Cells

Chondrogenic Differentiation Induction of Adipose-derived Stem Cells by Centrifugal Gravity

Rapid Isolation of BMPR-IB+ Adipose-Derived Stromal Cells for Use in a Calvarial Defect Healing Model

A Novel Mammary Fat Pad Transplantation Technique to Visualize the Vessel Generation of Vascular Endothelial Stem Cells

Horizontal Whole Mount: A Novel Processing and Imaging Protocol for Thick, Three-dimensional Tissue Cross-sections of Skin

In Vivo Tracking of Human Adipose-derived Mesenchymal Stem Cells in a Rat Knee Osteoarthritis Model with Fluorescent Lipophilic Membrane Dye

Single-cell Photoconversion in Living Intact Zebrafish

Computational Analysis of the Caenorhabditis elegans Germline to Study the Distribution of Nuclei, Proteins, and the Cytoskeleton

Read Article