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Immunomagnetic Separation of Fat Depot-specific Sca1high Adipose-derived Stem Cells (ASCs)
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Entwicklungsbiologie
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JoVE Journal Entwicklungsbiologie
Immunomagnetic Separation of Fat Depot-specific Sca1high Adipose-derived Stem Cells (ASCs)

Immunomagnetic Separation of Fat Depot-specific Sca1high Adipose-derived Stem Cells (ASCs)

6,772 Views

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08:52 min

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August 11, 2016

DOI:

10.3791/53890-v

DOI:
10.3791/53890-v

•

08:52 min
August 11, 2016

•

4 Views
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1Department of Internal Medicine, Division of Metabolism, Endocrinology and Diabetes,Biointerfaces Institute, University of Michigan

Summary

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We present the techniques required to isolate the stromal vascular fraction (SVF) from mouse inguinal (subcutaneous) and perigonadal (visceral) adipose tissue depots to assess their gene expression and collagenolytic activity. This method includes the enrichment of Sca1high adipose-derived stem cells (ASCs) using immunomagnetic cell separation.

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Immunomagnetic SeparationSca1high Adipose-derived Stem CellsFat Depot-specificMesenchymal Stem CellsSubcutaneous FatVisceral FatCollagenaseCell Sorting

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Barnes II, R. H., Chun, T. Immunomagnetic Separation of Fat Depot-specific Sca1high Adipose-derived Stem Cells (ASCs). J. Vis. Exp. (114), e53890, doi:10.3791/53890 (2016).

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