Acoustofluidic devices use ultrasonic waves within microfluidic channels to manipulate, concentrate and isolate suspended micro and nanoscopic entities. This protocol describes the fabrication and operation of such a device supporting bulk acoustic standing waves to focus particles in a central streamline without the aid of sheath fluids.
Shields IV, C. W., Cruz, D. F., Ohiri, K. A., Yellen, B. B., Lopez, G. P. Fabrication and Operation of Acoustofluidic Devices Supporting Bulk Acoustic Standing Waves for Sheathless Focusing of Particles. J. Vis. Exp. (109), e53861, doi:10.3791/53861 (2016).