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Immunology and Infection

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JoVE Journal Immunology and Infection
An Efficient and High Yield Method for Isolation of Mouse Dendritic Cell Subsets
 

An Efficient and High Yield Method for Isolation of Mouse Dendritic Cell Subsets

Article DOI: 10.3791/53824-v • 09:09 min • April 18th, 2016
April 18th, 2016 •
1, 1,2
1Department of Microbiology and Immunology, Albert Einstein College of Medicine, 2Department of Medicine (Rheumatology), Albert Einstein College of Medicine

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Distinct dendritic cell subsets exist as rare populations in lymphoid organs, and therefore are challenging to isolate in sufficient numbers and purity for immunological experiments. Here we describe a high efficiency, high yield method for isolation of all of the currently known major subsets of mouse splenic dendritic cells.

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Efficient High Yield Isolation Mouse Dendritic Cell Subsets Protocol Phenotypic Functional Divergence Filter Ligand Frequency Isolation Technique Commercially Available Agents Harvest Flt-3 Ligand Expressing B16 Melanoma Cells Culture Trypsin Protease Activity Complete Dima Medium Adherent Cell Model Layer Centrifugation Single Cell Suspension Subcutaneous Injection C57 Black 6 Recipient Mice Tumor Bearing Animals Spleens Petri Dish RPMI Medium
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