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Первичный энтодермальная эпителиальной клетки культуры из желтка мембраны японского перепела Эмбрионы
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Biologia do Desenvolvimento
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JoVE Journal Biologia do Desenvolvimento
Primary Endodermal Epithelial Cell Culture from the Yolk Sac Membrane of Japanese Quail Embryos
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Первичный энтодермальная эпителиальной клетки культуры из желтка мембраны японского перепела Эмбрионы

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11:53 min

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March 10, 2016

DOI:

10.3791/53624-v

DOI:
10.3791/53624-v

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11:53 min
March 10, 2016

•

8 Views
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1Department of Animal Science and Technology,National Taiwan University

Summary

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To study the mechanism of lipid utilization in yolk sac membranes during the late stages of avian embryonic development, we established a primary Japanese quail embryonic endodermal epithelial cell culture system.

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Endodermal Epithelial CellYolk Sac MembraneJapanese Quail EmbryosLipid UtilizationAvian EmbryogenesisDMEM/F-12Newborn Calf SerumPenicillin Streptomycin AmphotericinEgg CandlerCapillary VesselsEgg ShellYolk Sac Membrane IsolationEctoderm Layer

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Lin, H. J., Wang, S. H., Pan, Y. H., Ding, S. Primary Endodermal Epithelial Cell Culture from the Yolk Sac Membrane of Japanese Quail Embryos. J. Vis. Exp. (109), e53624, doi:10.3791/53624 (2016).

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