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Primary Endodermal Epithelial Cell Culture from the Yolk Sac Membrane of Japanese Quail Embryos
JoVE Journal
Entwicklungsbiologie
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JoVE Journal Entwicklungsbiologie
Primary Endodermal Epithelial Cell Culture from the Yolk Sac Membrane of Japanese Quail Embryos
DOI:

11:53 min

March 10, 2016

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Kapitel

  • 00:05Titel
  • 01:03Preparation of Basal Medium and Wash Solutions
  • 01:57Collection of Primary Endodermal Epithelium from Yolk Sac Membranes
  • 05:06Digestion of Endoderm by Collagenase
  • 08:26Results: Culture System Performance and its Application to Study Lipid Utilization
  • 10:30Conclusion

Summary

Automatische Übersetzung

To study the mechanism of lipid utilization in yolk sac membranes during the late stages of avian embryonic development, we established a primary Japanese quail embryonic endodermal epithelial cell culture system.

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