Rivista
/
Biologia dello sviluppo
/
Primary Endodermal Epithelial Cell Culture from the Yolk Sac Membrane of Japanese Quail Embryos
JoVE Journal
Biologia dello sviluppo
È necessario avere un abbonamento a JoVE per visualizzare questo.  Accedi o inizia la tua prova gratuita.
JoVE Journal Biologia dello sviluppo
Primary Endodermal Epithelial Cell Culture from the Yolk Sac Membrane of Japanese Quail Embryos

Primary Endodermal Epithelial Cell Culture from the Yolk Sac Membrane of Japanese Quail Embryos

DOI:
10.3791/53624-v

•

11:53 min

•

March 10, 2016

•

, , ,
1Department of Animal Science and Technology,National Taiwan University

Capitoli

  • 00:05Titolo
  • 01:03Preparation of Basal Medium and Wash Solutions
  • 01:57Collection of Primary Endodermal Epithelium from Yolk Sac Membranes
  • 05:06Digestion of Endoderm by Collagenase
  • 08:26Results: Culture System Performance and its Application to Study Lipid Utilization
  • 10:30Conclusion

Summary

Traduzione automatica

Traduzione automatica

To study the mechanism of lipid utilization in yolk sac membranes during the late stages of avian embryonic development, we established a primary Japanese quail embryonic endodermal epithelial cell culture system.

Tags

Avian Endodermal Epithelial CellsYolk Sac MembraneJapanese Quail EmbryosEx Vivo Explant ProcedureNutrient UtilizationLipid UtilizationYolk LipidsAvian EmbryogenesisFunctional Endodermal Epithelial CellsAvian Embryonic Development10x PBS SolutionGrowth MediumEECsDMEM/F-12Newborn Calf SerumPenicillin Streptomycin Amphotericin SolutionPH MeterHCl AdjustmentFertilized Quail Eggs

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

check_url/it/53624?article_type=v

Efficient Gene Transfer in Chick Retinas for Primary Cell Culture Studies: An Ex-ovo Electroporation Approach
check_url/it/53624?article_type=v

Cultivate Primary Nasal Epithelial Cells from Children and Reprogram into Induced Pluripotent Stem Cells
check_url/it/53624?article_type=v

Isolation and Characterization of Single Cells from Zebrafish Embryos
check_url/it/53624?article_type=v

Large-Scale Production of Cardiomyocytes from Human Pluripotent Stem Cells Using a Highly Reproducible Small Molecule-Based Differentiation Protocol
check_url/it/53624?article_type=v

Affinity Labeling Detection of Endogenous Receptors from Zebrafish Embryos
check_url/it/53624?article_type=v

Biosensing Motor Neuron Membrane Potential in Live Zebrafish Embryos
check_url/it/53624?article_type=v

Whole-cell Patch-clamp Recordings of Isolated Primary Epithelial Cells from the Epididymis
check_url/it/53624?article_type=v

Derivation of Stem Cell Lines from Mouse Preimplantation Embryos
check_url/it/53624?article_type=v

AFM and Microrheology in the Zebrafish Embryo Yolk Cell
check_url/it/53624?article_type=v

Application of Aorta-gonad-mesonephros Explant Culture System in Developmental Hematopoiesis

Read Article