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Photoconvertible PSmOrange 시스템을 사용하여 GFP 유전자 변형 Zebrafish의 세포를 추적
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Biologia do Desenvolvimento
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JoVE Journal Biologia do Desenvolvimento
Tracking Cells in GFP-transgenic Zebrafish Using the Photoconvertible PSmOrange System
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Photoconvertible PSmOrange 시스템을 사용하여 GFP 유전자 변형 Zebrafish의 세포를 추적

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07:59 min

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February 05, 2016

DOI:

10.3791/53604-v

DOI:
10.3791/53604-v

•

07:59 min
February 05, 2016

•

2 Views
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1Medical Faculty Mannheim, Department of Cell and Molecular Biology,Heidelberg University, 2COS and Nikon Imaging Center at the University of Heidelberg,Heidelberg University, 3Excellenzcluster CellNetworks,University of Heidelberg

Tags

PSmOrangePhotoconversionGFPZebrafishCell TrackingConfocal MicroscopyBiologia do DesenvolvimentoRegenerationTumor ProgressionInvasion488 Nm Laser561 Nm LaserZ-stackRegion Of InterestPhotoactivation BleachingAcquisition StimulationPhotoconversion Protocol

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Beretta, C. A., Dross, N., Engel, U., Carl, M. Tracking Cells in GFP-transgenic Zebrafish Using the Photoconvertible PSmOrange System. J. Vis. Exp. (108), e53604, doi:10.3791/53604 (2016).

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