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NMRによるリアルタイム酵素反応速度測定のための溶解動的核分極計装
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JoVE Journal Química
Dissolution Dynamic Nuclear Polarization Instrumentation for Real-time Enzymatic Reaction Rate Measurements by NMR
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NMRによるリアルタイム酵素反応速度測定のための溶解動的核分極計装

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10:54 min

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February 23, 2016

DOI:

10.3791/53548-v

DOI:
10.3791/53548-v

•

10:54 min
February 23, 2016

•

10 Views
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1LCBPT – UMR8601, Institut de Chimie,Université Paris Descartes, 2Institute of Physics of Biological Systems,EPFL, 3PARCC – INSERM U970,Université Paris Descartes

Summary

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The sensitivity enhancement provided by dissolution dynamic nuclear polarization (DNP) enables following metabolic processes in real time by NMR and MRI. The characteristics and performances of a dedicated dissolution DNP setup designed for study enzymatic reactions are discussed.

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Balzan, R., Fernandes, L., Comment, A., Pidial, L., Tavitian, B., Vasos, P. R. Dissolution Dynamic Nuclear Polarization Instrumentation for Real-time Enzymatic Reaction Rate Measurements by NMR. J. Vis. Exp. (108), e53548, doi:10.3791/53548 (2016).

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